Evaluation of nanoformulations on in vitro development of fungal phytopathogens

Zormy Nacary Correa-Pacheco, Silvia Bautista-Baños, Mónica Hernández-López, Miguel Ángel Marquina-Valle

Abstract


For controlling phytopathogenic microorganisms, there exist new proposals of nanomaterials including those from chitosan and essential oils, which may be applied alone or in coating nanoformulations. Six fungal isolates were subjected to different nanoparticles treatments in order to evaluate their effect on mycelial growth and spore germination. It was observed a total growth inhibition (100%) in most fungi when they were incubated on nutrient media amended with nanoparticles of chitosan loaded with thyme essential oil at 5%, applied either alone or in nanostructured coatings. As for the spore germination tests, a notable inhibition (50 % and 100%) was observed at 1% concentration of thyme essential oil depending on the isolate. When fungi were grown on individually treatments, germination was delayed by 6 h but completely stopped when incubated on coated nanoformulations. Compared to the lime essential oil, it was the thyme essential oil which provided a remarkable control of the tested fungi.


Keywords


nanomaterials; chitosan; essential oils

Full Text:

PDF (Español)

References


Barrera-Necha LL, Garduño-Pizaña G and García-Barrera J. 2009. In vitro antifungal activity of essential oils and their compounds on mycelial growth of Fusarium oxisporum f. sp. gladioli (Massey) Snyder and Hensen. Plant Pathology Journal 8:17-21. Disponible en línea: http://www.docsdrive.com/pdfs/ansinet/ppj/2009/17-21.pdf

Beyki M, Zhaveh S, Khalili TS, Rahmani-Cherati T, Abollahi A, Mansour B, Bayat Tabatabaei M and Mohsenifarc A. 2014. Encapsulation of Mentha piperita essential oils in chitosan–cinnamic acid nanogel with enhanced antimicrobial activity against Aspergillus flavus. Industrial Crops and Products 54:310-319. https://doi.org/10.1016/j.indcrop.2014.01.033

Correa-Pacheco ZN, Bautista-Baños S, Marquina-Valle MA and Hernández-López M. 2017. The effect of nanostructured chitosan and chitosan-thyme essential oil coatings on Colletotrichum gloeosporioides growth in vitro and on cv Hass and fruit quality. Journal of Phytopathology 165:297-305. DOI: 10.1111/jph.12562

Foladori G. 2009. La gobernanza de las nanotecnologías. Sociológica 24:125-153. Disponible en línea: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0187-01732009000300006

Khalili ST, Mohsenifar A, Beyki M, Zhaveh S. Rahmani-Cherati T, Abdollahi A, Bayat M and Tabatabaei M. (2015) Encapsulation of Thyme essential oils in chitosan-benzoic acid nanogel with enhanced antimicrobial activity against Aspergillus flavus. LWT-Food Science and Technology 60:502-508. https://doi.org/10.1016/j.lwt.2014.07.054

Mohammadi A, Nazari H, Imani S and Amrollahi H. 2014. Antifungal activities and chemical composition of some medicinal plants Activite´s antifongiques et composition chimique de quelques plantes médicinales. Journal of Mycologie Médicale 24:e1-e8. http://dx.doi.org/10.1016/j.mycmed.2014.02.006

Pekmezovic M, Rajkovic K, Barac A, Senerovic´ L and Arsic Arsenijevic V. 2015. Development of kinetic model for testing antifungal effect of Thymus vulgaris L. and Cinnamomum cassia L. essential oils on Aspergillus flavus spores and application for optimization of synergistic effect. Biochemical Engineering Journal 99:131-137. https://doi.org/10.1016/j.bej.2015.03.024

Saharan V1, Mehrotra A, Khatik R, Rawal P, Sharma SS and Pal A. 2013. Synthesis of chitosan based nanoparticles and their in vitro evaluation against phytopathogenic fungi. International Journal of Biological Macromolecules 62:677-683. https://doi.org/10.1016/j.ijbiomac.2013.10.012

Sellamuthu P, Sivakumar D and Soundy P. 2013. Antifungal activity and chemical composition of thyme, peppermint and citronella oils in vapor phase against avocado and peach postharvest pathogens. Journal of Food Safety 33:86-93. DOI: 10.1111/jfs.12026

Sivakumar D and Bautista-Baños S. 2014. A review on the use of essential oils for postharvest decay control and maintenance of fruit quality during storage. Crop Protection 64:27-37. http://dx.doi.org/10.1016/j.cropro.2014.05.012

Sotelo BME, Bautista BS, Aldana LL, Solorza FJ, Jiménez AA, Barrera NLL, Valverde AG y Plascencia JM. 2015. La nanotecnología en el control de microorganismos patógenos e insectos de importancia económica. In: C. Laréz VC, Koteich KS y López GF eds. Nanotecnología: fundamentos y aplicaciones. Comisión de Publicaciones del Departamento de Química de la Facultad de Ciencias, Universidad de los Andes, Caracas Venezuela 307-321.

Sotelo-Boyás M, Correa-Pacheco Z, Bautista-Baños S, Gómez and Gómez Y. 2017. Release study and inhibitory activity of thyme essential oil-loaded chitosan nanoparticles and nanocapsules against foodborne bacteria. International Journal of Biological Macromolecules 103:409-414. http://dx.doi.org/10.1016/j.ijbiomac.2017.05.063

Yien L, Mohammad N, Sarwar A and Katas H. 2012. Antifungal activity of chitosan nanoparticles and correlation with their physical properties. International Journal of Biomaterials 2012:1-9. http://dx.doi.org/10.1155/2012/632698

Zahid N, Alderson P, Ali A, Maqbool M and Manickam S. 2013. In vitro control of Colletotrichum gloeosporioides by using chitosan loaded nanoemulsions. Acta Horticulturae 1012:769-774. DOI: 10.17660/ActaHortic.2013.1012.104




DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1803-2

Refbacks

  • There are currently no refbacks.