Identification of Tomato brown rugose fruit virus by RT-PCR from a coding region of replicase (RdRP)

Johan Rodríguez-Mendoza, Clemente de Jesús García-Ávila, José Abel López-Buenfil, Karina Araujo-Ruiz, Andrés Quezada-Salinas, José Manuel Cambrón-Crisantos, Daniel Leobardo Ochoa-Martínez

Abstract


Currently, it is of strategic importance to have fast and accurate diagnostic tools that allow us to know if the material of interest is infected with a quarantine or economic important virus. For this reason in the present work we designed a pair of specific oligonucleotides for the detection of ToBRFV by RT-PCR, considering a region of the coding sequence of the RdRP located in the ORF1 and the methodology to detect it was standardized. Additionally, the amplicons were cloned and sequenced, the products were used to predict the evolutionary relationship between ToMMV, ToMV, TMV and ToBRFV by the Maximum Likelihood method. The results indicated that the oligonucleotides designed in the present work allows the identification of fast and specific ToBRFV.

Keywords


Solanaceae; Tobamovirus; specific oligonucleotides; standardization

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References


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DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1902-6

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