Fast method applied in previous evaluation of resistance of banana to Fusarium oxysporum f. sp. cubense

Rómulo García-Velasco, Nayanci Portal-González, Ramón Santos-Bermúdez, Ermis Yanes-Paz, José C. Lorenzo-Feijoo, Barbarita Companioni-González

Abstract


In previous works, a method to differentiate at leaf level the resistance of banana cultivars to Fusarium wilt was developed. The present study was carried out to evaluate such method by using fungus culture filtrates from Fusarium oxysporum f. sp. cubense race 1 VCG [01210] and race 2 VCG [0124/125] strains. Fungus culture filtrates were obtained during in vitro growth of the pathogen and the response of Musa spp. cultivars to fungus culture filtrates was determined. The 15- and 29-days-old host-specific culture filtrates from both races 1 and race 2 strains were able to differentiate more than 93% of individuals with a known response to the pathogen in vivo. We demonstrated that resistance or susceptibility to banana Fusarium wilt could be differentiated with the use of culture filtrates from diverse population of Fusarium oxysporum f. sp. cubense and a reproducible bioassay on banana detached leaves. Such results could be applicable in both conventional and biotechnological Musaceae breeding program.

Keywords


resistance selection; fungus filtrates; disease; Musa spp

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References


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DOI: http://dx.doi.org/10.18781/R.MEX.FIT.2004-1

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