Antagonistic bacteria for biospace control of roselle spot (Corynespora cassiicola) of Hibiscus sabdariffa

Alberto Patricio-Hernández, Santo Ángel Ortega-Acosta, Arturo Ramírez-Peralta, Alejandro Ayala-Sánchez, Francisco Palemón-Alberto, Erubiel Toledo-Hernández, Yanet Romero-Ramírez, Jeiry Toribio-Jiménez

Abstract


In recent years, Corynespora cassiicola induced spotted of roselle threatens calyx production. The objective was to determine if some bacterial strains inhibit the development of C. cassiicola in vitro and under biospace conditions. The inhibition of development was due to the dual confrontation of the bacteria: food (20), amphibian skin (41), tailings (22), air (seven) and rhizosphere (10) against the fungus. Plants of jamaican “Creole” variety were sown and artificially inoculated with C. cassiicola. From the beginning of symptoms of the disease, the selected bacterial strains with the highest percentage of inhibition were inoculated. AUDPC was evaluated. Only seven strains inhibited the development of the fungus in vitro from 62.7 to 100%: Klebsiella pneumoniae (M10-1 and M10-10), Acinetobacter lwoffii (A5), Sphingomonas paucimobilis (NF21), Serratia marcescens (M13ACD), S. liquefaciens (M8ACD) and Acinetobacter sp. (5H2). Regarding leaf severity, the M10-1 and M10-10 strains reduced AUDPC by 9.5 and 12.6%, respectively, followed by NF21 (AUDPC 14.5), the control registered the highest levels (AUDPC 45.7). For the severity in calyces, the AUDPC analyzes of all the strains exercised control by reducing severity levels (17.1-8.6 AUDPC).

Keywords


biological control; bacterial; phytopathogenic fungus; in vitro

Full Text:

PDF

References


Alejo JA. 2017. Jamaica (monocultivo). Pp: 23-29. In: Moctezuma LG, González HA, Romero SME, Pérez MR, Castillo MCR. (Eds.). Agenda Técnica Agrícola de Guerrero. INIFAP. 116 p. https://vun.inifap.gob.mx/VUN_MEDIA/BibliotecaWeb/_media/_agendas/4130_4827_Agenda_T%c3%a9cnica_Guerrero_2017.pdf

Blakeman JP and Fokkema NJ. 1982. Potential for biological control of plant diseases on the phylloplane. Annual Review of Phytopathology 20:167-92. https://doi.org/10.1146/annurev.py.20.090182.001123

Campbell CL and Madden LV. 1990. Introduction to Plant Disease Epidemiology. Jonh Wiley & Sons. New York, USA. 532p. https://doi.org/10.1017/S00074853000

Cuervo-Parral JA, Ramírez-Suerol M, Sánchez-López V. and Ramírez-Lepel M. 2011. Antagonistic effect of Trichoderma harzianum VSL291 on phytopathogenic fungi isolated from cocoa (Theobroma cacao L.) fruits. African Journal of Biotechnology 10(52):10657–10663. https://doi.org/10.5897/ajb11.1333

Dey S, Dutta P and Majumbar S. 2019. Biological control of Macrosphomina phaseolina in Vigna nungo L. by endophytic Klebsiella pneumoniae HR1. Jordan Journal of Biological Sciences 12(2): 210-227. http://jjbs.hu.edu.jo/files/vol12/n2/Paper%20number%2014.pdf

Dixon LJ, Schlub RL, Pernezny K, Datnoff LE. 2009. Host specialization and phylogenetic diversity of Corynespora cassiicola. Phytopathology 99(9): 1015-1027. https://doi.org/10.1094/PHYTO-99-9-1015

Durairaj K, Velmurugan P, Park JH, Chang WS, Park YJ, Senthilkumar P, Choi KM, Lee JH and Oh BT. 2018. An investigation of biocontrol activity Pseudomonas and Bacillus strains against Panax ginseng root rot fungal phytopathogens. Biological Control 125:138-146. https://doi.org/10.1016/j.biocontrol.2018.05.021

Fortunato AA, Debona D, Bernardeli AMA and Rodrigues FA. 2015. Changes in the antioxidant system in soybean leaves infected by Corynespora cassiicola. Phytopathology 105: 1050-1058. https://doi.org/10.1094/PHYTO-10-14-0283-R

Ghosal A and Hati A. 2019. Impact of some new generation insecticides on soil arthropods in rice maize cropping system. The Journal of Basic and Applied Zoology 80(6). https://doi.org/10.1186/s41936-019-0077-3

Hahn M. 2014. The rising threat of fungicide resistance in plant pathogenic fungi: Botrytis as a case study. Journal of Chemical Biology 7(4):133-141. https://doi.org/10.1007/s12154-014-0113-1

Hernández-Morales J, Ochoa-Martínez DL, Ortega-Acosta, SÁ and Vega-Muñoz R. 2018. Survey on alternative hosts of Corynespora cassiicola, the cause of the leaf and calyx spot, in the surroundings of roselle fields in Mexico. Tropical Plant Pathology 43: 263-270. https://doi.org/10.1007/s40858- 017-0206-9

Köhl J, Kolnaar R and Ravensberg WJ. 2019. Mode of Action of Microbial Biological Control Agents Against Plant Diseases: Relevance Beyond Efficacy. Frontiers in Plant Science 10:845. https://doi.org/10.3389/fpls.2019.00845

Manju MJ, Sadananda M, Santhosh HM, Roopa S, Patil TH, Shankarappa VI, Benagi and Idicula P. 2019. Evaluation of different fungi toxicants against Corynespora cassiicola causing Corynespora Leaf Fall (CLF) disease of rubber [Hevea brasiliensis Muell. Arg.,] International Journal of Current Microbiology and Applied Sciences 8(2): 1640-1647. https://doi.org/10.20546/ijcmas.2019.802.193

Medina-De la Rosa G, López-Reyes L, Carcaño-Montiel MG, López-Olguín JH, Hernández-Espinosa MA and Rivera-Tapia JA. 2016. Rhizosphere bacteria of maize with chitinolytic activity and its potential in the control of phytopathogenic fungi. Archives of Phytopathology and Plant Protection 49 (12): 310-321. https://doi.org/10.1080/03235408.2016.1201345

Mustafa S, Kabir S, Shabbir U and Batool R. 2019. Plant growth promoting rhizobacteria in sustainable agriculture: from theoretical to pragmatic approach. Symbiosis 78: 115-123. https://doi.org/10.1007/s13199-019-00602-w

Nghia NA, Kadir J, Sunderasan E, Puad AM, Malik A and Napis S. 2008. Morphological and inter simple sequence repeat (ISSR) markers analyses of Corynespora cassiicola isolates from rubber plantations in Malaysia. Mycopathologia 166: 189-201. https://doi.org/10.1007/s11046-008-9138-8

Ortega-Acosta SÁ, Mora-Aguilera JA, Velasco-Cruz C, Ochoa-Martínez DL, Leyva-Mir SG and Hernández- Morales J. 2020a. Temporal progress of roselle (Hibiscus sabdariffa L.) leaf and calyx spot disease (Corynespora cassiicola) in Guerrero, Mexico. Journal of Plant Pathology. https://doi.org/10.1007/s42161-020-00550-1

Ortega-Acosta SA, Ochoa-Martínez DL, Hernández-Morales J and Palemón-Alberto F. 2020b. Morphological and genetic characterization of Corynespora cassiicola isolates obtained from roselle and associated weeds. Mexican Journal of Phytopathology 38(1): 62-78. https://doi.org/10.18781/r.mex.fit.1909-2

Ortega-Acosta SA, Velasco-Cruz C, Hernández-Morales J, Ochoa-Martínez DL and Hernández-Ruiz J. 2016. Diagrammatic logarithmic scales for assess the severity of spotted leaves and calyces of roselle. Mexican Journal of Phytopathology 34(3): 270-285. https://doi.org/10.18781/r.mex.fit.1606-6

Ortega-Acosta SA, Ochoa-Martínez DL, Leyva-Mir SG, Velasco- Cruz C, Mora-Aguilera JA y Hernández-Morales J. 2019. Control químico del manchado de hojas y cálices de jamaica en Guerrero, México. Summa Phytopathologica, 45(1): 38-43. https://doi.org/10.1590/0100-5405/182006

Ortega-Acosta SA, Hernández-Morales L, Ochoa-Martínez D and Ayala-Escobar V. 2015. First report of Corynespora cassiicola causing leaf and calyx spot on roselle in Mexico. Plant Disease 99(7): 1041. https://doi.org/10.1094/pdis-04-14-0438-pdn

Petatán-Sagahón I, Anducho-Reyes MA, Silva-Rojas HV, Arana-Cuenca A, Tellez-Jurado A, Cárdenas-Álvarez IO and Mercado-Flores Y. 2011. Isolation of bacteria with antifungal activity against the phytopathogenic fungi Stenocarpella maydis and Stenocarpella macrospora. International Journal of Molecular Sciences 12(9): 5522-5537. https://doi.org/10.3390/ijms12095522

Qi Y-X, Zhang X, J-J P, Liu X-M, Lu Y, Zhang H, Hui-Qiang Z, YanChao L and Yi-Xian X. 2011. Morphological and molecular analysis of genetic variability within isolates of Corynespora cassiicola from different hosts. European Journal of Plant Pathology 130:83-95. https://doi.org/10.1007/s10658-010-9734-6

Rahman MZ, Khanam H, Ueno M, Kihara J, Honda Y and Arase S. 2010. Suppression by red light irradiation of Corynespora leaf spot of cucumber caused by Corynespora cassiicola. Journal of Phytopathology 158: 378-381. https://doi.org/10.1111/j.1439-0434.2009.01632.x

Romeiro RS, Lana R, Macagnan D, Garcia F and Silva H. 2010. Evidence that the biocontrol agent Bacillus cereus synthesizes protein that can elicit increased resistance of tomato leaves to Corynespora cassiicola. Tropical Plant Pathology 35(1): 011-015. https://doi.org/10.1590/s1982-56762010000100002

Sabu R, Aswani R, Jishma P, Jasim B, Mathew J and Radhakrishnan EK. 2017. Plant growth promoting endophytic Serratia sp. ZoB14 protecting ginger from fungal pathogens. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. https://doi.org/10.1007/s40011-017-0936-y

Shekhar S, Sundaramanickam A and Balasubramanian T. 2015. Biosurfactant producing microbes and their potential applications: a review. Critical Review in Environmental Science and Technology 45:1522-1554. https://doi.org/10.1080/10643389.2014.955631.

SIAP. 2018. Estadística de Producción Agrícola. Sistema de Información Agrícola y Pesquera. http://infosiap.siap.gob.mx/gobmx/datosAbiertos_a.php.

Trotel-Aziz P, Couderchet M, Biagianti S and Aziz A. 2008. Characterization of new bacterial biocontrol agents Acinetobacter, Bacillus, Pantoea and Pseudomonas spp. Mediating grapevine resistance against Botrytis cinerea. Environmental and Experimental Botany 64: 21-32. https://doi.org/10.1016/j.envexpbot.2007.12.009

Vargas-Jordá L, Vila A, Lanza A, Bonvehi P, Nazar J, Mikietuk A, Labat R y Smayevsky J. 2005. Utilidad del sistema VITEK en la identificación bacteriana y estudios de sensibilidad antimicrobiana Acta Bioquímica Clínica Latinoamericana 39 (1): 19-25.

Vawdrey LL, Grice KRE and Westerhuis D. 2008. Field and laboratory evaluations of fungicides for the control of brown spot (Corynespora cassiicola) and black spot (Asperisporium caricae) of papaya in far north Queensland, Australia. Australasian Plant Pathology 37: 552-558. https://doi.org/10.1071/AP08055

Villanueva-Couoh E, Sánchez-Briceño MA, Cristóbal-Alejo J, Ruiz-Sánchez E y Tún-Suárez J M. 2005. Diagnóstico y alternativas de manejo químico del tizón foliar (Alternaria chrysanthemi Simmons y Crosier) del crisantemo (Chrysanthemum morifolium Ramat.) kitamura en Yucatán, México. Revista Mexicana de Fitopatología 23(1): 49-56. https://www.redalyc.org/pdf/612/61223107.pdf

Weber RWS and Hahn M. 2019. Grey mould disease of strawberry in northern Germany: causal agents, fungicide resistance and management strategies. Applied Microbiology and Biotechnology 103(4):1589?1597. https://doi.org/10.1007/s00253-018-09590-1

Zhu J, Zhang L, Li T, Ma D, Gao Y, Mu W and Liu F. 2020. Baseline sensitivity of Corynespora cassiicola to metconazole and efficacy of this fungicide. Crop Protection 130: 105056. https://doi.org/10.1016/j.cropro.2019.105056




DOI: http://dx.doi.org/10.18781/R.MEX.FIT.2006-1

Refbacks

  • There are currently no refbacks.