Population dynamics of the carrot cyst nematode Heterodera carotae under greenhouse conditions

Ilia Mariana Escobar-Ávila, Alejandro Tovar-Soto

Abstract


The carrot cyst nematode Heterodera carotae is an important pest of Daucus carota crops. For control measures to be effective, knowledge about nematode population dynamics over time is essential. This study evaluated the population dynamics of H. carotae in carrot over a ten-month cultivation period under greenhouse conditions. In June 2017, 90 pots (3 L volume) were placed in the greenhouse: 60 were filled with nematode naturally infested soil (1200 J2/pot) and the remaining 30 were controls (without the nematode). Ten seeds of carrot were sown into each pot. After germination, pots were destructively sampled every 15 and 30 days (n=3). Roots from each pot were weighed and stained. J2s and cysts were extracted from soil. J2 populations in soil and roots behaved similarly. In soil, three J2 peaks were observed after 105, 150 and 195 days with 735, 882 and 967 J2/200 cm3 of soil, respectively. A multiplication factor of 1.6 was calculated and five generations of the nematode were observed. H. carotae caused an average 70.8% yield loss during the experiment. The results can be used in the development of decision-making tools to optimize the implementation of management measures.

Keywords


cyst-forming nematode; Daucus carota; multiplication factor; life cycle; generations; endo-radicular stages

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References


Ambrogioni L and Marinari-Palmisano A. 1976. Effetto di avvicendamenti colturali su Heterodera carotae (Nematoda: Heteroderidae) e sulla produzione di carota in terreno infestato. Redia 59: 355.

Aubert V. 1986. Hatching of the carrot cyst nematode. Pp: 347–348. In: Lamberti F and Taylor CE (eds.). Cyst nematodes. Plenum Press, London, UK. 467 p. https://doi.org/10.1007/978-1-4613-2251-1_26

Baldwin JG and Mundo-Ocampo M. 1991. Heteroderinae, cyst- and non-cyst forming nematodes. Pp: 275-362. In: Nickle WR (ed.). Manual of Agricultural Nematology. Marcel Dekker, Inc, New York, USA. 1064 p.

Barrot L. 2018. Resistance to Heterodera carotae and methods for use. US Patent 20,180,103,647. April 19.

Byrd DWJr, Kirkpatrick T and Barker KR, 1983. An improved technique for clearing and staining plant tissues for detection of nematodes. Journal of Nematology 15(1): 142–143. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2618249/

Davis RM and Nuñez J. 2007. Integrated Approach for Carrot Pest and Disease Management. Pp: 149-188. In: Ciancio A and Mukerji KG (eds.). General Concepts in Integrated Pest and Disease Management. Springer, The Netherlands. https://doi.org/10.1007/978-1-4020-6061-8_6

Escobar-Avila IM, Medina-Canales MG and Tovar-Soto A. 2017. Distribution, life cycle and histological changes by Heterodera sp. in carrot in Puebla. Mexican Journal of Phytopathology 35(2): 304-313. https://doi.org/10.18781/r.mex.fit.1606-7

Escobar-Avila IM, López-Villegas EO, Subbotin SA and Tovar-Soto A. 2018. First Report of Carrot Cyst Nematode Heterodera carotae in Mexico: Morphological, Molecular Characterization and Host Range Study. Journal of Nematology 50(2): 229-242. https://doi.org/10.21307/jofnem-2018-021

FAO, 2020. Food and Agriculture organization. http://www.fao.org/faostat/. (Review september, 2020).

Fenwick DW. 1940. Methods for the recovery and counting of cysts of Heterodera schachtii from soil. Journal of Helminthology 18: 155-172. https://doi.org/10.1017/S0022149X00031485

Greco N. 1986. “The carrot cyst nematode”. Pp: 333-346. In: Lamberti F and Taylor CE (eds.). Cyst nematodes. Plenum Press, London, UK. 467 p. https://doi.org/10.1007/978-1-4613-2251-1_25

Greco N. 1987. Heterodera carotae: A destructive nematode of carrot. Nematology Circular No. 140. Florida Department of Agriculture and Consumer Services, Division of Plant Industry. Gainesville, Florida, USA. 4 p.

Greco N and Brandonisio A. 1980. Relationship between Heterodera carotae and carrot yield. Nematologica 26(4): 497-500. https://doi.org/10.1163/187529280x00440

Greco N and Brandonisio A. 1986. The Biology of Heterodera carotae. Nematologica 32(4): 447-460. https://doi.org/10.1163/187529286x00327

Greco N, D´Addabbo T, Brandonisio A and Elia F. 1993. Damage to Italian crops caused by cyst-forming nematodes. Journal of Nematology 25 (4S): 836-842. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2619465/

Groves RL, Clements JR and Bradford BZ. 2020. Carrot diseases resulting from phytoplasmas and viruses. Pp: 148-155. In: Geoffriau E and Simon P (eds). Carrots and Related Apiaceae Crops. CAB International, London, UK. 368 p. https://doi.org/10.1079/9781789240955.0148

Hajihasani A, Tanha Maafi Z, Nicol JM and Rezaee S. 2010. Effect of the cereal cyst nematode, Heterodera filipjevi on wheat in microplot trials. Nematology 12(3): 357-363. https://doi.org/10.1163/138855409x12548945788321

Handoo ZA and Subbotin SA. 2018. Taxonomy, Identification and Principal Species. Pp: 365-398. In: Perry RN, Moens M and Jones JT (eds.). Cyst nematodes. CAB International, London, UK. 464 p. https://doi.org/10.1079/9781786390837.0365

Jenkins DW. 1964. A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Disease Reporter 48(9): 692.

Jones FGW. 1950a. A new species of root eelworm attacking carrots. Nature 165: 81. https://doi.org/10.1038/165081a0

Jones FGW. 1950b. Observations on the beet eelworm and other cyst-forming species of Heterodera. Annals of Applied Biology 37(3): 407–440. https://doi.org/10.1111/j.1744-7348.1950.tb00966.x

Mugniery D and Bossis M. 1988. Heterodera carotae Jones, 1950. 1. Gamme d’hôtes, vitesse de développement, cycle. Revue de Nématologie 11(3): 307-313. https://core.ac.uk/reader/39867740

Schomaker CH and Been TH. 2006. Plant Growth and Population Dynamics. Pp: 275-301. In: Perry RN and Moens M (eds.). Plant Nematology. CAB International, London, UK. 463 p. https://doi.org/10.1079/9781845930561.0275

Seinhorst JW. 1966. Longidorus elongatus on Fragaria vesca. Nematologica 12(2): 275-279. https://doi.org/10.1163/187529266x00680

Seinhorst JW. 1986. Effects of nematode attack on the growth and yield of crop plants. Pp: 191-209. In: Lamberti F and Taylor CE (eds.). Cyst nematodes; Plenum Press, London, UK. 467 p. https://doi.org/10.1007/978-1-4613-2251-1_11

Seo Y, Kim YS, Park Y and Kim YH. 2015. Comparisons of Pathological Responses in Carrot to Root-knot Nematodes. The Plant Pathology Journal 31(4): 441-445. http://dx.doi.org/10.5423/PPJ.NT.06.2015.0115

SIAP, 2020. Secretaria de Información Agroalimentaria y Pesquera. https://nube.siap.gob.mx/cierreagricola/. (Review september, 2020).

Subbotin SA, Mundo-Ocampo M and Baldwin JG. 2010. Systematics of cyst nematodes (Nematoda: Heteroderinae). Nematology monographs and perspectives. Vol. B. Brill, The Netherlands. 512 p. https://doi.org/10.1163/ej.9789004164345.i-512

Winslow RD. 1955. The hatching responses of some root eelworms of the genus Heterodera. Annals of Applied Biology 43: 19-36. https://doi.org/10.1111/j.1744-7348.1955.tb02450.x

Yu Q, Ponomareva E, Van Dyk D, McDonald MR, Sun F, Madani M, Tenuta M, 2017. First report of the carrot cyst nematode (Heterodera carotae Jones) from carrot fields in Ontario, Canada. Plant Disease 101(6): 1056. https://doi. org/10.1094/pdis-01-17-0070-pdn




DOI: http://dx.doi.org/10.18781/R.MEX.FIT.2007-1

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