Identification and molecular analysis of races of Fusarium oxysporum f. sp. lycopersici isolated from tomato in Baja California, Mexico

Sara Elodia Armenta-López, Cesar Valenzuela-Solano, Rufina Hernández-Martínez

Abstract


In Baja California, Fusarium oxysporum f. sp. lycopersici (Fol) is the causal agent of vascular wilt of tomato; however, the races present in the state remain unknown. The objective of this work was to isolate and identify Fusarium spp. and to validate a multiplex-PCR system to identify strains Fol and at the race level. Sixty symptomatic plants were collected, of which 45 isolates were obtained. When analyzed microscopically, it was confirmed that 44 corresponded to Fusarium spp. The use of the sp13 and sp23 oligonucleotide pairs that amplified the pgx4 and pg1 polygalacturonase genes, respectively, indicated the presence of Fol races 1 and 3 in the area. Sequence analysis of the elongation factor of the 1? translation (TEF1-?) of representative strains served to confirm its identity and the presence of Fol and F. solani in tomato plants with symptoms of vascular wilt. Finally, a RAPD analysis showed that the oligonucleotide OPA-11, generates a specific banding pattern in Fol race 1, so it could be used as a quick way to identify them.

Keywords


DNA Polymorphisms; xylem; vascular disease; fungi

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References


Ascencio-Álvarez A, López-Benítez A, Borrego-Escalante F, Rodríguez-Herrera SA, Flores-Olivas A, Jiménez-Díaz F y Gámez-Vázquez, AJ. 2008. Marchitez vascular del jitomate: I. Presencia de razas de Fusarium oxysporum f. sp. lycopersici (Sacc.) Snyder y Hansen en Culiacán, Sinaloa, México. Revista Mexicana de Fitopatología 26(2):114- 120. https://www.redalyc.org/articulo.oa?id=61226203

Assigbetse KB, Fernández D, Dubois MP and Geiger JP. 1994. Differentiation of Fusarium oxysporum f. sp. vasinfectum races on cotton by Random Amplified Polymorphic DNA (RAPD) analysis. Phytopathology 84: 622-626. https://www.apsnet.org/publications/phytopathology/backissues/Documents/1994Articles/Phyto84n06_622.PDF

Baysal Ö, Siragusa M, Ikten H, Polat I, Gümrükcü E, Yigit F, Carimi JA and da Silva JT. 2009. Fusarium oxysporum f. sp. lycopersici races and their genetic discrimination by molecular markers in West Mediterranean region of Turkey. Physiological and Molecular Plant Pathology 74(1): 68-75. https://doi.org/doi:10.1016/j.pmpp.2009.09.008

Baysal Ö., Siragusa M, Gumrukcu E, Zengin S, Carimi F, Sajeva M and Teixeira da Silva, JA. 2010. Molecular characterization of Fusarium oxysporum f. melongenae by ISSR and RAPD markers on eggplant. Biochemical genetics 48(5-6):524–37. https://doi.org/doi:10.1007/s10528-010-9336-1

Bentley S, Pegg KG and Dale JL. 1994. Optimization of RAPD?PCR fingerprinting to analyse genetic variation within populations of Fusarium oxysporum f. sp. cubense. Journal of Phytopathology 142:164-78. https://doi.org/doi:10.1111/j.1439-0434.1994.tb00008.x

Biju VC, Fokkens L, Houterman PM, Rep M and Cornelissen BJ. 2017. Multiple evolutionary trajectories have led to the emergence of races in Fusarium oxysporum f. sp. lycopersici. Applied and environmental microbiology 83(4). https://doi.org/10.1128/AEM.02548-16

Cai G, Gale LR, Schneider RW, Kistler HC, Davis RM, Elias KS and Miyao EM. 2003. Origin of race 3 of Fusarium oxysporum f. sp. lycopersici at a single site in California. Phytopathology 93(8):1014-1022. https://doi.org/10.1094/PHYTO.2003.93.8.1014

Carrillo Fasio JA, Montoya Rodríguez TJ, García Estrada RS, Cruz Ortega JE, Márquez Zequera I y Sañudo Barajas AJ. 2003. Razas de Fusarium oxysporum f. sp. lycopersici. Snyder y Hansen en jitomate (Lycopersicon esculentum Mill) en el valle de Culiacán, Sinaloa, México. Revista Mexicana de Fitopatología 21:123-127. https://www.redalyc.org/articulo.oa?id=61221205.

Chang YD. Du B, Wang L, Ji P, Xie Y J, Li X F and Wang JM. 2018. A study on the pathogen species and physiological races of tomato Fusarium wilt in Shanxi, China. Journal of Integrative Agriculture 17:1380-1390. https://doi.org/10.1016/S2095-3119(18)61983-5

Gonçalves AM, Costa H, Fonseca MEN, Boiteux LS, Lopes CA and Reis A. 2016. Variability and geographical distribution of Fusarium oxysporum f. sp. lycopersici physiological races and field performance of resistant sources in Brazil. In V International Symposium on Tomato Diseases: Perspectives and future directions in tomato protection 1207 (pp. 45-50). https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/3237/1/goncalves2018.pdf

Grajal MJ, Simon CJ and Muehlbauer FJ. 1993. Use of Random Amplified Polymorphic DNA (RAPD) to characterize race 2 of Fusarium oxysporum f. sp. pisi. Phytopathology 83:612-614. https://www.apsnet.org/publications/phytopathology/backissues/Documents/1993Articles/Phyto83n06_612.PDF

Hernández Martínez R, López Benítez A, Borrego Escalante F, Espinoza Velázquez J, Sánchez Aspeytia D, Maldonado Mendoza IE y López Ochoa LA. 2014. Razas de Fusarium oxysporum f. sp. lycopersici en predios jitomateros en San Luis Potosí. Revista Mexicana de Ciencias Agrícolas 5(7):1169-1178. https://cienciasagricolas.inifap.gob.mx/editorial/index.php/agricolas/article/view/838/661

Hirano Y and Arie T. 2006. PCR-based differentiation of Fusarium oxysporum ff. sp. lycopersici and radicis-lycopersici and races of F. oxysporum f. sp. lycopersici. Journal of General Plant Pathology 72(5):273-283. https://doi.org/10.1007/s10327-006-0287-7

Holguín-Peña RJ. 2005. Fusarium wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici race 3 in Baja California Sur, México. Plant Disease 89(12):1360-1360. https://doi.org/10.1094/PD-89-1360C

Isaac MR and Leyva-Mir SG, Sahagun-Castellanos J, Camara- Correia K, Tovar-Pedraza JM y Rodriguez-Perez JE. 2018. Occurrence, identification, and pathogenicity of Fusarium spp. associated with tomato wilt in Mexico. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 46(2):484-493. https://doi.org/10.15835/nbha46211095

Jiménez Gasco MM, Pérez E and Jiménez RM. 2001. Identification of pathogenic races 0, 1B/C, 5, and 6 of Fusarium oxysporum f. sp. ciceris with random amplified polymorphic DNA (RAPD). European Journal of Plant Pathology 107:237-248. https://doi.org/10.1023/A:1011294204630

Katkar M and Mane SS. 2012. Characterization of Indian races of Fusarium oxysporum f. sp ciceris through RAPD markers. International Journal of Agriculture, Environment and Biotechnology 5(4):323-328. https://www.indianjournals.com/ijor.aspx?target=ijor:ijaeb&volume=5&issue=4&article=003

Kawabe M, Kobayashi Y, Okada G, Yamaguchi I, Teraoka T and Arie T. 2005. Three evolutionary lineages of tomato wilt pathogen, Fusarium oxysporum f. sp. lycopersici, based on sequences of IGS, MAT1, and pg1, are each composed of isolates of a single mating type and a single or closely related vegetative compatibility group. Journal of General Plant Pathology 71(4):263-272. https://doi.org/10.1007/s10327-005-0203-6

Kumar S, Stecher G, Li M, Knyaz C and Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35:1547-1549. https://doi.org/10.1093/molbev/msy096

Lievens B, Van Baarlen P, Verreth C, Van Kerckhove S, Rep M and Thomma BP. 2009. Evolutionary relationships between Fusarium oxysporum f. sp. lycopersici and F. oxysporum f. sp. radicis-lycopersici isolates inferred from mating type, elongation factor-1? and exopolygalacturonase sequences. Mycological research 113(10):1181-1191. https://www.sciencedirect.com/science/article/abs/pii/S0953756209001452

Leslie JF and Summerell BA. 2008. The Fusarium laboratory manual. John Wiley & Sons.

Lin YH, Chen KS, Chang JY, Wan YL, Hsu CC, Huang JW and Chang PFL. 2010. Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp. niveum in Taiwan. New Biotechnology 27(4):409-18. https://doi.org/10.1016/j.nbt.2010.05.005

Luna-Paez A, Silva-Rojas HV, Marbán-Mendoza N y Valadez-Moctezuma E. 2004. Variabilidad genética de Fusarium oxysporum Schlechtend.: Fr. f. sp. ciceris (Padwick) Matuo y K. Sato mediante PCR-RAPD’S en el Bajío de México. Revista Mexicana Fitopatología 22:44-51. http://scielo.sld.cu/scielo.php?pid=S1562-30092009000300004&script=sci_abstract

Martel MB, Létoublon R and Fèvre M. 1998. Purification and characterization of two endopolygalacturonases secreted during the early stages of the saprophytic growth of Sclerotinia sclerotiorum. FEMS microbiology letters 158(1):133-138. https://doi.org/10.1111/j.1574-6968.1998.tb12812.x

Migheli Q, Briatore E and Garibaldi A. 1998. Use of random amplified polymorphic DNA (RAPD) to identify races 1, 2, 3 and 8 of Fusarium oxysporum f. sp. dianthi in Italy. European Journal of Plant Pathology. 104:49-57. https://doi.org/10.1023/A:1008682328764

Murugan L, Krishnan N, Venkataravanappa V, Saha S, Mishra AK, Sharma BK and Rai AB. 2020. Molecular characterization and race identification of Fusarium oxysporum f. sp. lycopersici infecting tomato in India. 3 Biotech 10(11):1-12. https://doi.org/10.1007/s13205-020-02475-z

O’Donnell K. Kistler HC, Cigelnik E and Ploetz RC. 1998. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences 95(5):2044-2049. https://doi.org/10.1073/pnas.95.5.2044

Pirayesh S, Zamanizade H and Morid B. 2018. Molecular Identification of Physiological Races of Fusarium oxysporum f. sp. lycopersici and radicis lycopersici Causal Agent of Fusarium Wilt of Tomato in Iran. Journal of Agricultural Science and Technology 20(1):193-202. https://jast.modares.ac.ir/article-23-4759-en.html

Shimazu J, Yamauchi N, Hibi T, Satou M, Horiuchi S and Shirakawa T. 2005. Development of sequence tagged site markers to identify races of Fusarium oxysporum f. sp. lactucae. Journal of General Plant Pathology 71(3):183–189. https://doi.org/10.1007/s10327-005-0183-6

SIAP, 2019. Recuperado 13 de octubre de 2020: http://infosiap.siap.gob.mx:8080/agricola_siap_gobmx/Resumen-Delegacion.do

Valenzuela-Ureta JG, Lawn DA, Heisey RF and Zamudio-Guzman V. 1996. First report of Fusarium wilt race 3, caused by Fusarium oxysporum f. sp. lycopersici, of tomato in Mexico. Plant Disease 80(1):105. https://www.apsnet.org/publications/plantdisease/backissues/Documents/1996Abstracts/PD_80_0105A.htm.

Vakalounakis DJ and Fragkiadakis GA. 1999. Genetic diversity of Fusarium oxysporum isolates from cucumber: Differentiation by Pathogenicity, Vegetative Compatibility, and RAPD Fingerprinting. Phytopathology 89(2):161-8. https://doi.org/10.1094/PHYTO.1999.89.2.161

Vawdrey LL and Peterson RA. 1988. Fusarium solani, the cause of foot rot of tomatoes in Central Queensland. Australasian Plant Pathology, 17(1):24-25. https://doi.org/10.1071/APP9880024




DOI: http://dx.doi.org/10.18781/R.MEX.FIT.2103-4

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