Molecular identification of bacteria associated to ornamental plants obtained in vitro

Sergio Ramírez-Rojas, Felipe de Jesus Osuna-Canizalez, Faustino García-Pérez, Jaime Canul-Ku, Jesús Hernández-Romano, Katya Ornelas-Ocampo, Patricia Landa-Salgado

Abstract


In ornamental plants propagated in vitro at Tezoyuca Technology Development Center, belonging to FIRA, symptoms of necrosis on leaves and stems during the adaptation stage. The objective of this study was to isolate and to identify the agents related to these plants. Eleven bacterial isolates were obtained from necrotic leaves and stems, from which ADN was extracted using a commercial PCR kit. The PCR products were sequenced and analyzed using the Chromas Lite® program. We search for homology with BLAST after which the following bacteria were identified: Kosakonia oryzae, Pectobacterium cypripedii, Burkholderia tropica, Serratia marcescens, Pantoea dispersa, Erwinia cypripedii, Pantoea agglomerans y Erwinia rhapontici. The last three bacteria are plant disease in phase of acclimation in ornamental plants get in vitro.


Keywords


Potted plant; Pathogens; Molecular identification; PCR

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References


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DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1511-3

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