Email this article In vitro and in vivo antifungal activity of Azadirachta indica and Piper auritum extracts against uredospore germination of Hemileia vastatrix
Abstract
Coffee (Coffea arabica) production is one of the most economically important industrial activities in México. However, coffee production faces phytosanitary problems in the form of coffee rust (Hemileia vastatrix). The main goal of the present study was to evaluate extracts from Azadirachta indica and Piper auritum as potential biofungicides. In in vitro assays, ethanolic and nonpolar foliar extracts from A. indica and P. auritum were applied in three concentrations 1000, 3000, and 5000 ppm, against H. vastatrix. While in the in vivo assay, only ethanolic extracts were applied to coffee plants Typica diseased with H. vastatrix. Both extracts inhibited about 99% of the germination of H. vastatrix uredospores in vitro. On coffee plants of “Typica” variety infected with H. vastatrix that were treated with the ethanolic extracts from P. auritum and A. indica, no disease appeared during the 20 days of treatment. The inhibitory effect was not significantly similar in those treated with copper oxychloride on days 15 and 20 (Tukey P ? 0.05). We suggest that the evaluated plant extracts are used as a natural alternative for the preventive control of H. vastatrix in small greenhouses.
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Alvarado-Castillo G, Benítez-Badillo G, Lozada-García JA, Ortiz-Ceballos G and Torres-Pelayo VR. 2017. Uredospores mycelium germination inhibition of coffee rust (Hemileia vastatrix) through three alternative compounds: First study. Wulfenia 24: 65-78. http://www.multidisciplinarywulfenia.org/auto/index.php/archive/part/24/2/1/?currentVol=24¤tissue=2
Avelino J, Cristancho M, Georgiou S, Imbach P, Aguilar L, Bornemann G, Läderach P, Anzueto F, Hruska AJ and Morales C. 2015. The coffee rust crises in Colombia and Central America (2008-2013): impacts, plausible causes and proposed solutions. Food Security 7: 303-321. https://doi.org/10.1007/s12571-015-0446-9
Avelino J and Rivas G. 2013. La roya anaranjada del cafeto. 1-47pp. https://hal.archives-ouvertes.fr/hal-01071036
Babatunde DE, Otusemade GO, Efeovbokhan VE, Ojewumi ME, Bolade OP and Owoeye TF. 2019. Chemical composition of steam and solvent crude oil extracts from Azadirachta indica leaves. Chemical Data Collections 20: 100208. https://doi.org/10.1016/j.cdc.2019.100208
Cantrell CL, Dayan FE and Duke SO. 2012. Natural products as sources for new Pesticides. Journal of Natural Products 75(6): 1231-1242. https://doi.org/10.1021/np300024u
Capucho AS, Zambolim L, Duarte HSS and Vaz GRO. 2011. Development and validation of a standard area diagram set to estimate severity of leaf rust in Coffea arabica and C. canephora. Plant Pathology 60: 1144-50. https://doi.org/10.1111/j.1365-3059.2011.02472.x
Durant-Archibold AA, Santana AI and Gupta MP. 2018. Ethnomedical uses and pharmacological activities of most prevalent species of genus Piper in Panama: A review. Journal of Ethnopharmacology 217: 63-82. https://doi.org/10.1016/j.jep.2018.02.008
FAO. 2015. Manejo agroecológico de la roya del café. Organización de las naciones unidas para la alimentación y la agricultura ciudad de panamá. 96 p. https://www.fao.org/documents/card/fr/c/a55342d5-58e1-4c57-b1bd-7f65cc80e547/
Guillén-Sánchez D, Pérez R, Andrade-Rodríguez M, López-Martínez V, Alia-Tejacal I and Juárez-López P. 2017. Eficacia de seis fungicidas en el control de Mycosphaerella citri Whiteside en naranja, Var., 'Valencia' en Tlayecac, Morelos, México. Centro Agrícola 44(3): 71-79. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-57852017000300010&lng=es&tlng=es
Gregorí-Valdés BS. 2005. Estructura y actividad de los antifúngicos. Revista Cubana de Farmacia, 39 (2): 1-15. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0034-75152005000200012&lng=es&tlng=es
Heidtmann-Bemvenuti R, Tralamazza SM, Jorge Ferreira CF, Corrêa B and Badiale-Furlong E. 2016. Effect of natural compounds on Fusarium graminearum complex. Journal of the Science of Food and Agriculture 96(12): 3998-4008. https://doi:10.1002/jsfa.7591
Hernández-Solabac JAM, Nava-Tablada ME, Díaz-Cárdenas S, Pérez-Portilla E and Escamilla-Prado E. 2011. Migración internacional y manejo del café en dos comunidades del Centro de Veracruz. Tropical and Subtropical Agroecosystems 14:807-818. http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-04622011000300013
Leahy J, Mendelsohn M, Kough J, Jones R and Berckes N. 2014. Biopesticide oversight and registration at the US Environmental Protection Agency. Biopesticides: state of the art and future opporunities. American Chemical Society. 3-18 p. https://doi:10.1021/bk-2014-1172.ch001
López-García FJ, Escamilla-Prado E, Zamarripa-Colmenero A and Cruz-Castillo JG. 2016. Producción y calidad en variedades de café (Coffea arabica) en Veracruz, México. Revista Fitotecnia Mexicana 39(3): 297-304. http://www.scielo.org.mx/pdf/rfm/v39n3/0187-7380-rfm-39-03-00297.pdf
Mesa VAM, Marín PA, Ocampo O, Calle J and Monsalve Z. 2019. Fungicidas a partir de extractos vegetales: una alternativa en el manejo integrado de hongos fitopatógenos. Revista de Investigaciones Agropecuarias 45(1): 23-30. https://www.redalyc.org/articulo.oa?id=86458941001
Mgbeahuruike EE, Yrjönen T, Vuorela H and Holm Y. 2017. Bioactive compound from medicinal plants: Focus on the Piper species. South African Journal of Botany 112: 54-69. https://doi.org/10.1016/j.sajb.2017.05.007
Mohammad RO, Ling Wang, Zhou qi Cui, Chunlan Yang, Tao Zhongyun and Guan-Lin Xie. 2014. Antifungal and SAR potential of crude extracts derived from neem and ginger against storage carrot rot caused by Sclerotinia Sclerotium. Industrial Crops & Products 55:130-139. https://doi.org/10.1016/j.indcrop.2014.02.012
Mohamed-Ali EO, Akhtar Shakil N, Singh Rana V, Sarkar DJ, Majumder S, Kaushik P, Bhushan Singh B and Kumar J. 2017. Antifungal activity of nano emulsions of neem and citronella oil against phytopathogenic fungi, Rhizoctonia solani and Sclerotium rolfsii. Industrial Crops & Products 108: 379-387. https://doi.org/10.1016/j.indcrop.2017.06.061
Mohanty SSG, Raghavendra K and Prasad Dash A. 2008. Influence of growth medium on antifungal activity of neem oil (Azadirachta indica) against Lagenidium giganteum and Metarhizium anisopliae. Mycoscience 49: 318-320. https://doi.org/10.1007/S10267-008-0425-5
Montes-Belmont R. 2009. Diversidad de compuestos químicos producidos por las plantas contra hongos fitopatógenos. Revista mexicana de micología 29: 73-82. http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0187-31802009000100010&lng=es
Nagano MS and Batalini C. 2021. Phytochemical screening, antioxidant activity and potential toxicity of Azadirachta indica A. Juss (neem) leaves. Revista Colombiana de Ciencias Químico - Farmacéuticas 50(1): 29-47. https://doi.org/10.15446/rcciquifa.v50n1.95447
Ordanza-Beneitez MA. 2017. Biopesticidas: Tipos y aplicaciones en el control de plagas agrícolas. Agroproductividad 10 (3): 31-367. https://revista-agroproductividad.org/index.php/agroproductividad/article/view/966
Pérez AP and Pérez TM. 2012. Las denominaciones de origen del café mexicano y sus cuestionamientos como modelo de desarrollo regional. Revista Perspectivas Rurales Nueva Época 10(3): 43-56. https://www.revistas.una.ac.cr/index.php/perspectivasrurales/article/view/3381
Pineda RM, Samuel Vizcaíno P, García MPP, Gil GGH and Durango DLR. 2012. Chemical composition and antifungal activity of Piper auritum and Piper holtonii Against phytopathogenic fungi. Chilean Journal of Agricultural Research 72(4): 507-515. http://dx.doi.org/10.4067/S0718-58392012000400008
Polanco-Florián LG, Alvarado-Gómez OG, Olivares-Sáenz E, González-Garza R and Pérez-González O. 2020. Control biológico de Lasiodiplodia theobromae y Fomitopsis meliae causantes de la muerte regresiva de los cítricos. Revista Mexicana de Ciencias Agrícolas 11 (5): 1069-1081. https://doi.org/10.29312/remexca.v11i5.2272
Rodríguez AT, Morales D and Ramírez MA. 2000. Efecto de extractos vegetales sobre el crecimiento in vitro de hongos fitopatógenos. Cultivos Tropicales 21(2): 79-82. https://www.redalyc.org/articulo.oa?id=193215024014
Salehi B, Zakaria ZA, Gyawali R, Ibrahim SA, Rajkovic J, Shinwari ZK, Khan T, Sharifi-Rad J, Ozleyen A, Turkdonmez E, Valussi M, Tumer TB, Monzote Fidalgo L, Martorell M and Setzer WN. 2019. Piper species: A comprehensive review of their phytochemistry, biological activity and aplications. Molecules 24(7): 1364. https://doi:10.3390/molecules24071364
Sánchez-Fernández ER, Sánchez-Ortiz BL, Sandoval-Espinosa YKM, Ulloa-Benítez A, Armendáriz-Guillén B, García-Méndez MC and Macías-Rubalcava ML. 2013. Hongos endófitos: fuente potencial de metabolitos secundarios bioactivos con utilidad en agricultura y medicina. Revista Especializada en Ciencias Químico-Biológicas 16 (2): 132-146. https://doi.org/10.1016/S1405-888X(13)72084-9
SAGARPA. 2015. Estudio de necesidades de innovación productiva para los cafeticultores del estado de Tabasco. 1-122 p. https://www.gob.mx/cms/uploads/attachment/file/346977/Cafeticultores_Ejecutivo.pdf
SENASICA. 2019. Roya del cafeto (Hemileia vastatrix). Dirección General de Sanidad Vegetal-Programa de Vigilancia Epidemiológica Fitosanitaria. Ciudad de México. Última actualización: mayo de 2019. Ficha Técnica No. 40. 1-16 p.
SINAVEF. 2013. Manual técnico para el manejo preventivo de la roya del cafeto. México. 26 p. http://www.royacafe.lanref.org.mx/Documentos/Manualtecnicoroya.pdf
Silva-Acuña R, Zambolim L and Alvarez-Venegas VH. 2002. Estrategias de control de la roya del cafeto con la aplicación de fungicida protector y sistémico en viçosa, minas gerais, Brasil. Bioagro 14(2): 85-97. https://www.redalyc.org/articulo.oa?id=85714205
Shigueoka LH, Hiroshi-Sera G, Sera T, de Batista Fonseca IC, Junior VM, Andreazi E, Gimenez-Carvalho F, Gonçalves-Gardiano C and Carducci FC. 2014. Selection of Arabic coffee progenies with rust resistance. Crop Breeding and Applied Biotechnology 14(2): 88-93. https://doi.org/10.1590/1984-70332014v14n2a16
Sparks TC, Hahn DR and Garizi NV. 2017. Natural products, their derivatives, mimics and synthetic equivalents: role in agrochemical discovery. Pest management science 73(4):700-715. https://doi.org/10.1002/ps.4458
Várzea VM and Marques DV. 2005. Population variability of Hemileia vastatrix vs coffee durable resistance. In: Zambolin L, Zambolim, EM, Várzea VM. (Eds.) Durable Resistance of Coffee Leaf Rust. Editora Universidade Federal de Viçosa Brasil. 53-74 p.
Villavicencio-Nieto MA and Pérez-Escandón BE. 2010. Plantas tradicionalmente usadas como plaguicidas en el Estado de Hidalgo, México. 30: 193-238. http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-27682010000200012&lng=es&tlng=es
Virginio-Filho EM and Astroga-Domian C. 2015. Prevención y control de la roya del café: manual de buenas prácticas para técnicos y facilitadores. 1°ed., Turrialba, C.R: CATIE. No. 131. 1-96p. http://repositorio.bibliotecaorton.catie.ac.cr/bitstream/handle/11554/8186/Prevencion_y_contol_de_la_roya_del_cafe.pdf
DOI: http://dx.doi.org/10.18781/R.MEX.FIT.2205-1
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