In vitro inhibition of Botrytis cinerea with extracts of wild grapevine (Vitis spp.) leaves

Isela Apolonio Rodríguez, Omar Franco Mora, Martha Lydia Salgado Siclán, Jesús Guadencio Aquino Martínez

Abstract


The extracts of wild grapevines (Vitis) leaves (EHVS) from three accessions (P- 178, E-200 and TN-4) at 6, 8 and 12 % v/v, were tested in vitro to evaluate their inhibitory effect on Botrytis cinerea development. Resveratrol (RVS) (60, 90, 120 µg/mL), citrus extracts (EC) (3, 5 and 8 % v/v) and cyprodinil + fludioxonil (SW) (500, 800 and 1000 µg/mL) were compared against EHVS. In average, SW presented inhibition of mycelial growth (ICM), sporulation (IE) and spore germination (IGC) (88.9; 85.5 and 93.7 %, average respectively). RVS presented the second higher inhibition rate. All EHVS presented antifungal activity; specially, P-178 at 12 % resulted in 72 % ICM, 75 % IE and 62 % IGC. This dose contained phenolic compounds 19.9 mg/mL, RVS 1.7 mg/mL, gallic acid 3.8 mg/mL and ferulic acid 2.5 mg/mL. 


Keywords


Ferulic acid; gallic acid; antifungal activity; phenolic compounds; resveratrol

Full Text:

PDF (Español)

References


Adrian M and Jeandet P. 2012. Effects of resveratrol on the ultrastructure of Botrytis cinerea conidia and biological significance in plant/pathogen interactions. Fitoterapia 83:1345-1350. http://dx.doi.org/10.1016/j.fitote.2012.04.004

Alonso-Villaverde V, Voinesco F, Viret O, Spring JL and Gindro K. 2011. The effectiveness of stilbenes in resistant Vitaceae: ultrastructural and biochemical events during Plasmopara viticola infection process. Plant Physiology and Biochemestry 49:265-274. http://dx.doi.org/10.1016/j.plaphy.2010.12.010

Barnett HL and Hunter BB. 1998. Illustrated genera of imperfect fungi. Ed. Minenesota US: Burgess Publishing Company, New York. 241 p.

Caruso F, Mendoza L, Castro P, Cotoras M, Aguirre M, Matsuhiro B, Isaacs M, Rossi M, Viglianti A and Antonioletti R. 2011. Antifungal activity of resveratrol against Botrytis is improved using 2-furyl derivatives PLOS ONE 6:e25421. http://dx.doi.org/doi:10.1371/journal.pone.0025421

Compean KL and Ynalvez RA. 2014. Antimicrobial activity of plant secondary metabolites: A review. Journal of Medicinal Plants Research 8:204-213. http://dx.doi.org/ 10.3923/rjmp.2014.204.213

Cotoras M, Mendoza L, Muñoz A, Yáñez K, Castro P and Aguirre M. 2011. Fungitoxicity against Botrytis cinerea of a flavonoid isolated from Pseudognaphalium robustum. Molecules 16:3885-3895. http://dx.doi.org/doi:10.3390/molecules16053885

Crous PW, Braun U and Groenewald JZ. 2007. Mycosphaerella is polyphyletic. Studies in Mycology 58:1-32. http://dx.doi.org/10.3114/sim.2007.58.01

Cruz, C. 2007. Las uvas (Vitis) silvestres: Distribución y usos en la región central de Veracruz, pp. 225-235. En: Nieto AR (Ed.): Frutales nativos, un recurso filogenético de México. Ed. Universidad Autónoma Chapingo, Chapingo, México.

Ellis MB. 1971. More dematiaceous hyphomycetes. Ed. Commonwealth Mycological Institute. Kew, Surrey, England. 608 pp. http://dx.doi.org/10.1007/BF01989814

El-Khateeb AY, Elsherbiny EA, Tadros LK, Ali SM and Hamed HB. 2013. Phytochemical analysis and antifungal activity of fruit leaves extracts on the mycelial growth of fungal plant pathogens. Journal of Plant Pathology and Microbiology 4:1-6. http://dx.doi.org/10.4172/2157-7471.1000199

Enríquez-Guevara EA, Aispuro-Hernández E, Vargas-Arispuro, I y Martínez-Téllez MA. 2010. Oligosacarinas derivadas de pared celular: Actividad biológica y participación en la respuesta de defensa de plantas. Revista Mexicana de Fitopatología 28: 144-155. http://www.scielo.org.mx/pdf/rmfi/v28n2/v28n2a7.pdf

Fernández-Ortuño D, Grabke A, Bryson PK, Beasley ED, Fall LA and Brannen PM. 2013. First report of fludioxonil resistance in Botrytis cinerea from a blackberry field in Georgia. Plant Disease 98:848. http://dx.doi.org/10.1094/PDIS-10-13-1020-PDN

Franco MO, Cruz CJG, González HA y Pérez LDJ. 2012. Distribución y caracterización. En: Franco MO, Cruz CJG. (Eds.): La vid silvestre en México. Actualidades y potencial, pp. 42-67. México: Universidad Autónoma del Estado de México-Altres-Costa Amic Editores. https://www.researchgate.net/publication/262014593_La_vid_silvestre_en_Mexico_Actualidades_y_potencial

Guerrero RF, Puertas B, Fernández MI, Palma M and Cantos-Villar E. 2010. Induction of stilbenes in grapes by UV-C: Comparison of different subspecies of Vitis. Innovative Food Science Emerging Technology 11:231-238. http://dx.doi.org/10.1016/j.ifset.2009.10.005

Katalinić V, Generalić I, Skroza D, Ljubenkov I, Teskera A, Konta I and Boban M. 2009. Insight in the phenolic composition and antioxidative properties of Vitis vinifera leaves extracts. Croatian Journal of Food Science and Technology 1:7-15. https://www.researchgate.net/publication/44201407

Lorrain B, Ky I, Pechamat L and Teissedre PL. 2013. Evolution of analysis of polyhenols from grapes, wines and extracts. Molecules 18:1076-1100. http://dx.doi.org/10.3390/molecules18011076

Mendoza L, Yánez K, Vivanco M, Melo R and Cotoras M. 2013. Characterization of extracts from winery by-products with antifungal activity against Botrytis cinerea. Industrial Crops and Products 43:360-364. http://dx.doi.org/10.1016/j.indcrop.2012.07.048

Minova S, Seðíçna R, Voitkâne S, Metla Z, Daugavietis M and Jankevica L. 2015. Impact of pine (Pinus sylvestris L.) and (Picea abies (L.) Karst.) bark extracts on important strawberry pathogens. Proceedings of the Latvian Academy of Sciences 69: 62-67. http://dx.doi.org/10.1515/prolas-2015-0008

Mora VHF, Franco-Mora OF, López-Sandoval JA, Pérez-López DJ y Balbuena-Melgarejo A. 2009. Characterization of wild plum (Ximenia americana L. var. americana; Olacaceae) fruit growing at Tepexi de Rodriguez, Puebla, Mexico. Genetic Resources and Crop Evolution 56:719-727. http://dx.doi.org/10.1007/s10722-009-9422-6

Moo-Koh FA, Alejo CJ, Reyes-Ramírez A, Tun-Suárez J M, Sandoval-Luna R y Ramírez-Pool JA. 2014. Actividad in vitro del extracto acuoso del Bonellia flammea contra hongos fitopatógenos. Agrociencia 48:833-845. http://www.colpos.mx/agrocien/Bimestral/2014/nov-dic/art-6.pdf

Panebianco A, Castello I, Cirvilleri G, Perrone G, Epifani F, Ferrara M, Polizzi G, Walters DR and Vitale A. 2015. Detection of Botrytis cinerea field isolates with multiple fungicide resistance from table grape in Sicily. Crop Protection 77:65-73. http://dx.doi.org/10.1016/j.cropro.2015.07.010

Piesik D, Miler N, Lemańczyk G, Bocianowski J and Buszewski B. 2015. Botrytis cinerea infection in three cultivars of chrysanthemum in ‘Alchimist’ and its mutants: Volatile induction of pathogen-infected plants. Scientia Horticulturae 193:127-135. http://dx.doi.org/10.1016/j.scienta.2015.06.040

Salgado M, Rodríguez-Rojo S, Alves-Santos FM and Cocero MJ. 2015. Encapsulation of resveratrol on lecithin and β-glucans to enhance its action against Botrytis cinerea. Journal of. Food Engineering 165:13-21. http://dx.doi.org/10.1016/j.jfoodeng.2015.05.002

SAS Institute. 2002. SAS/STAT software: Version 9.0. SAS Institute Inc. Cary, NC.

Schnee S, Queiroz EF, Voinesco F, Marcourt L, Dubuis P. H, Wolfender JL and Gindro K 2013. Vitis vinifera Canes, a new source of antifungal compounds against Plasmopara viticola, Erysiphe necator, and Botrytis cinerea. Journal of Agricultural and Food Chemistry 61:5459-5467. http://dx.doi.org/10.1021/jf4010252

Soylu EM, Kurt Åž and Soylu S. 2010. In vitro and in vivo antifungal activities of the essential oils of various plants against tomato grey mould disease agent Botrytis cinerea. International Journal of Food Microbiology 143:183-189. http://dx.doi.org/10.1016/j.ijfoodmicro.2010.08.015

Tobar-Reyes JR. Franco-Mora O, Morales-Rosales EJ y Cruz-Castillo JG. 2009. Contenido de resveratrol en hojas de vides silvestres (Vitis spp.) mexicanas. Revista de la Facultad de Ciencias Agrarias 41: 127-37. www.redalyc.org/articulo.oa?id=382837645010

Tobar-Reyes JR. Franco-Mora O, Morales-Rosales EJ y Cruz-Castillo JG. 2011. Fenoles de interés farmacológico en vides silvestres (Vitis spp.) de México. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas 10:167-172. www.redalyc.org/articulo.oa?id=85617384011

Vio-Michaelis S., Apablaza-Hidalgo G, Gómez M, Pena-Vera R and Montenegro G. 2012. Antifungal activity of tree Chilean plant extracts on Botrytis cinerea. Botanical Sciences 90:179-183. http://dx.doi.org/10.17129/botsci.482

Wu CF, Yang JY, Wang F and Wang X. 2013. Resveratrol: botanical origin, pharmacological activity and applications. Chinese Journal of Natural Medicines 11:1-15. http://dx.doi.org/10.1016/S1875-5364(13)60001-1

Xu W-T, Huang K-L, Guo F, Qu W, Yang JJ, Liang Z-H and Luo YB. 2007. Postharvest grapefruit seed extract and chitosan treatments of table grapes to control Botrytis cinerea. Postharvest Biology and Technology 46:86-94. http://dx.doi.org/10.1016/j.postharvbio.2007.03.019

Yadav IC, Devi NL, Syed JH, Cheng Z, Li J, Zhang G and Jones KC. 2015. Current status of persistent organic pesticides residues in air, water, and soil, and their possible effect on neighboring countries: A comprehensive review of India. Science of the Total Environment 511:123-137. http://dx.doi.org/10.1016/j.scitotenv.2014.12.041




DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1611-1

Refbacks

  • There are currently no refbacks.