• Vol. 2025, 43(3)
• Phytopathological Note

Diversity of endophytic bacteria in strawberry plants (Fragaria sp.) in Sinaloa

By Rosa M Longoria Espinoza*, Rubén Félix Gastélum, Dulce R Hernández Luna

*Corresponding Author.

Received: 09/August/2024 – Published: 30/August/2025
DOI: https://doi.org/10.18781/R.MEX.FIT.2408-2

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  • Longoria-Espinoza RM, Félix-Gastélum R, Hernández-Luna DR. 2025. Diversity of endophytic bacteria in strawberry plants (Fragaria sp.) in Sinaloa. Mexican Journal of Phytopathology 43(3):86. https://doi.org/10.18781/R.MEX.FIT.2408-2
  • Longoria-Espinoza, RM, Félix-Gastélum, R y Hernández-Luna, DR (2025). Diversity of endophytic bacteria in strawberry plants (Fragaria sp.) in Sinaloa. Mexican Journal of Phytopathology 43(3):86. https://doi.org/10.18781/R.MEX.FIT.2408-2
  • Rosa Longoria-EspinozaM, Rubén Félix-Gastélum y Dulce Hernández-LunaR. 2025. Diversity of endophytic bacteria in strawberry plants (Fragaria sp.) in Sinaloa. Mexican Journal of Phytopathology 43(3):86. https://doi.org/10.18781/R.MEX.FIT.2408-2

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Abstract Background/Objective. Currently, agricultural systems require management strategies based on crop sustainability. Plant growth-promoting bacteria are an environmentally friendly option to reduce the use of chemicals in agriculture and promote sustainable agricultural practices. The objective of this research was to isolate and identify endophytic bacteria in strawberry plants (Fragaria x ananassa) cv Chanler, grown under organic conditions and to evaluate in vitro their ability to produce metabolites related to plant growth promotion.

Materials and Methods. Twenty-five healthy-looking strawberry plants of the Chanler variety were collected during the fall-winter 2023-2024 cycle. Genomic DNA was isolated from samples composed of root, stem, and leaf tissue, and the 16S rDNA gene was amplified.

Results. Analysis of genomic sequences identified bacterial genera associated with the phylum Firmicutes, with the genus Bacillus predominating, along with the species B. cereus; B. subtilis, B. thuringiensis, B. xiamenensis, and B. zanthoxyli. Shimwellia blattae and Burkholderia tropica were detected in smaller numbers. Of these isolates, B. cereus, B. subtilis, and B. thuringiensis, in that order, showed the ability to produce siderophores, chitinase, and solubilize phosphorus in vitro.

Conclusion. B. cereus, B. subtilis, and B. thuringiensis exhibited metabolic characteristics related to plant growth promotion in vitro. These results in the existence of a diversity of endophytic bacteria in commercial strawberries. This provides information for understanding the endophytic bacteria-plant interaction in the specific environment of strawberry cultivation in the northern state of Sinaloa.

Keywords: Siderophores, Phosphates, Chitinase, B. cereus.

Table 1. Qualitative in vitro siderophore, chitinase, and phosphate production activity of six species of endophytic bacteria isolated from Chanler strawberry plants grown under organic production. Municipality of Ahome, Sinaloa, Mexico.

Figure 1. In vitro colonial morphology of endophytic bacteria isolated from the roots, stems, leaves, and fruit of strawberry plants (Fragaria x ananassa) cv. Chanler, viewed a digital stereoscope after 48 h of growth on nutrient agar. Note: The different morphology: smooth, translucent edges, circular shape, shiny surface, and coloration.

Figure 2. Dendrogram obtained using the Neighbor-Joining algorithm from sequences of the gene encoding the 16S rDNA subunit obtained from endophytic bacteria isolated from strawberry plants (Fragaria x ananassa), cv. Chanler, from organic commercial crops. Municipality of Ahome, Sinaloa, Mexico. The isolates obtained in this research are identified with acronyms and numbers: R-n: root; T-n: stem; H-n: leaf; F-n: fruit.