• Vol. 2025, 43(3)
• Phytopathological Report

First report of Orthotospovirus impatiensnecromaculae infecting lettuce (Lactuca sativa) in Guanajuato, Mexico

By María de los Ángeles Mora Ugalde, Jesús Serrano Castillejos, Alfredo Diaz Lara*

*Corresponding Author.

Received: 26/March/2025 – Published: 16/July/2025
DOI: https://doi.org/10.18781/R.MEX.FIT.2503-3

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  • Mora-Ugalde M, Serrano-Castillejos J, Diaz-Lara A. 2025. First report of Orthotospovirus impatiensnecromaculae infecting lettuce (Lactuca sativa) in Guanajuato, Mexico. Mexican Journal of Phytopathology 43(3):76. https://doi.org/10.18781/R.MEX.FIT.2503-3
  • Mora-Ugalde, M, Serrano-Castillejos, J y Diaz-Lara, A (2025). First report of Orthotospovirus impatiensnecromaculae infecting lettuce (Lactuca sativa) in Guanajuato, Mexico. Mexican Journal of Phytopathology 43(3):76. https://doi.org/10.18781/R.MEX.FIT.2503-3
  • María de los Ángeles Mora-Ugalde, Jesús Serrano-Castillejos y Alfredo Diaz-Lara. 2025. First report of Orthotospovirus impatiensnecromaculae infecting lettuce (Lactuca sativa) in Guanajuato, Mexico. Mexican Journal of Phytopathology 43(3):76. https://doi.org/10.18781/R.MEX.FIT.2503-3

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Abstract Background/Objective. Determine the presence of Orthotospovirus impatiensnecromaculae (Impatiens necrotic spot virus, INSV) in lettuce crops in Guanajuato, Mexico.

Materials and Methods. Symptomatic lettuce samples were collected from a commercial field in Guanajuato, Mexico, in May 2024. The detection and identification in 12 individual samples was performed with INSV ImmunoStrips and reverse transcription-PCR (RT-PCR) and corroborated by Sanger sequencing.

Results. Ten of the 12 lettuce samples tested positive for O. impatiensnecromaculae by ImmunoStrips. The identity of the virus was confirmed through RT-PCR and Sanger sequencing (GenBank: PQ151798).

Conclusion. The presence of INSV in lettuce crops from Guanajuato, Mexico highlights the need for continuous monitoring and implementation of integrated disease management strategies to mitigate the spread and impact in this region.

Keywords: Impatiens necrotic spot virus, ImmunoStrips, RT-PCR, Trips

Figure 1. Symptomatic lettuce plants infected by Orthotospovirus impatiensnecromaculae observed during the field survey.

Figure 2. Maximum-likelihood phylogenetic tree of Orthotospovirus impatiensnecromaculae (Impatiens necrotic spot virus, INSV) isolates available in GenBank. Sequence generated during this study is indicated with red color. GenBank: S48091 corresponds to Orthotospovirus tomatomaculae (tomato spotted wilt virus, TSWV) and is included in the phylogenetic analysis as outgroup.