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First detection of bell pepper endornavirus infecting bell pepper in Guanajuato, Mexico. Figure 1 - Phylogenetic tree constructed using the Maximum Likelihood method based on the bell pepper endornavirus (BPEV, <em>Alphaendornavirus capsici</em>) polyprotein sequence and other Alphaendornavirus sequences available in GenBank. Reference sequences were selected from GenBank based on their annotation as BPEV isolates and sequence availability. The analysis was performed using the corresponding nucleotide substitution model. Bootstrap support values (1,000 replicates) are indicated at the nodes, showing only those greater than 50%. Sequence PZ103604 (indicated by an arrow) clusters with closely related sequences with high bootstrap support, suggesting a close phylogenetic relationship. Sequence DQ399290 (<em>Betaendornavirus</em>) was used as the outgroup. The scale bar represents 0.20 substitutions per site.

Figure 1 - Phylogenetic tree constructed using the Maximum Likelihood method based on the bell pepper endornavirus (BPEV, Alphaendornavirus capsici) polyprotein sequence and other Alphaendornavirus sequences available in GenBank. Reference sequences were selected from GenBank based on their annotation as BPEV isolates and sequence availability. The analysis was performed using the corresponding nucleotide substitution model. Bootstrap support values (1,000 replicates) are indicated at the nodes, showing only those greater than 50%. Sequence PZ103604 (indicated by an arrow) clusters with closely related sequences with high bootstrap support, suggesting a close phylogenetic relationship. Sequence DQ399290 (Betaendornavirus) was used as the outgroup. The scale bar represents 0.20 substitutions per site.

Epidemiology of coffee leaf rust under the effect of fungicides in the Northern and Northeastern Sierra of Puebla state. Figure 3 - Effect of fungicides on the dynamics of rust (<em>H. vastatrix</em>) severity in coffee plants grown in Huehuetla, Ixtepec, and Zongozotla, Puebla. A) Lipuntahuaca 1 (Costa Rica 95), B) Lipuntahuaca 2 (Typica), C) Escatachuchut 1 (Costa Rica 95), D) Escatachuchut 2 (Garnica), E) Zongozotla 1 (Marsellesa), and F) Zongozotla 2 (Oro Azteca). Arrows indicate the timing of fungicide application: orange: Azoxystrobin, gray: <em>M. alternifolia</em>, and green: <em>B. subtilis</em>. The asterisk (*) indicates significant differences between treatments at each sampling point, according to Tukey's mean comparison test.

Figure 3 - Effect of fungicides on the dynamics of rust (H. vastatrix) severity in coffee plants grown in Huehuetla, Ixtepec, and Zongozotla, Puebla. A) Lipuntahuaca 1 (Costa Rica 95), B) Lipuntahuaca 2 (Typica), C) Escatachuchut 1 (Costa Rica 95), D) Escatachuchut 2 (Garnica), E) Zongozotla 1 (Marsellesa), and F) Zongozotla 2 (Oro Azteca). Arrows indicate the timing of fungicide application: orange: Azoxystrobin, gray: M. alternifolia, and green: B. subtilis. The asterisk (*) indicates significant differences between treatments at each sampling point, according to Tukey's mean comparison test.

Use of <em>Purpureocillium lilacinum</em> MTL01 and <em>Bacillus velezensis</em> 83 to reduce galling by <em>Meloidogyne enterolobii</em> and improve flowering in tomato. Figure 3 - The combination of <em>P. lilacinum</em> MTL01 and <em>B. velezensis</em> 83 promotes flowering and reduces galling in tomato plants infected with M. enterolobii. <strong>A</strong>), <strong>C</strong>), and <strong>E</strong>): Flower counts throughout the life cycle before the end of each experiment. <strong>B</strong>), <strong>D</strong>), and <strong>F</strong>): Root galling by experiment. Different letters indicate statistically significant differences (ANOVA, Tukey’s test; p < 0.05).

Figure 3 - The combination of P. lilacinum MTL01 and B. velezensis 83 promotes flowering and reduces galling in tomato plants infected with M. enterolobii. A), C), and E): Flower counts throughout the life cycle before the end of each experiment. B), D), and F): Root galling by experiment. Different letters indicate statistically significant differences (ANOVA, Tukey’s test; p < 0.05).

Epidemiology of coffee leaf rust under the effect of fungicides in the Northern and Northeastern Sierra of Puebla state. Figure 2 - Temperature and relative humidity in Huehuetla, Puebla. Period: April 2024 to March 2025. The gray band indicates the optimal temperature range for coffee leaf rust (17 to 26°C), and the green band indicates the optimal relative humidity range (80–100%) reported by SENASICA (2019) and Parada-Molina <em>et al</em>. (2020).

Figure 2 - Temperature and relative humidity in Huehuetla, Puebla. Period: April 2024 to March 2025. The gray band indicates the optimal temperature range for coffee leaf rust (17 to 26°C), and the green band indicates the optimal relative humidity range (80–100%) reported by SENASICA (2019) and Parada-Molina et al. (2020).

Epidemiology of coffee leaf rust under the effect of fungicides in the Northern and Northeastern Sierra of Puebla state. Figure 5 - Effect of fungicides on the yield of coffee grown in Huehuetla, Ixtepec, and Zongozotla, Puebla. A) Lipuntahuaca 1: Costa Rica 95, B) Lipuntahuaca 2: Typica, C) Escatachuchut 1: Costa Rica 95, D) Escatachuchut 2: Garnica, E) Zongozotla 1: Marsellesa, and F) Zongozotla 2: Oro Azteca. The bars indicate the treatments: TE = Control, BS = <em>Bacillus subtilis</em>, MA = <em>Melaleuca alternifolia</em>, and AZ = Azoxystrobin. Bars with the same letter are statistically equal (Tukey, p≤0.05).

Figure 5 - Effect of fungicides on the yield of coffee grown in Huehuetla, Ixtepec, and Zongozotla, Puebla. A) Lipuntahuaca 1: Costa Rica 95, B) Lipuntahuaca 2: Typica, C) Escatachuchut 1: Costa Rica 95, D) Escatachuchut 2: Garnica, E) Zongozotla 1: Marsellesa, and F) Zongozotla 2: Oro Azteca. The bars indicate the treatments: TE = Control, BS = Bacillus subtilis, MA = Melaleuca alternifolia, and AZ = Azoxystrobin. Bars with the same letter are statistically equal (Tukey, p≤0.05).

Histopathology of <em>Peronospora tabacina</em> in tobacco. Figure 1 - Stages of the <em>Peronospora tabacina</em> infection process in artificially inoculated tobacco leaves. A) Transversal section of a healthy leaf displaying the abaxial (EAb) and adaxial (EAd) epidermis and stomata (St). B) Longitudinal section of a healthy leaf displaying the epidermis (Ep), vascular bundles (Hv) and palisade parenchyma (Pe). C) Longitudinal section 24 hai with sporangia (Es) in the trichome base. D) Longitudinal section 48 hai with sporangia in the epidermis. Bar = 40 µm.

Figure 1 - Stages of the Peronospora tabacina infection process in artificially inoculated tobacco leaves. A) Transversal section of a healthy leaf displaying the abaxial (EAb) and adaxial (EAd) epidermis and stomata (St). B) Longitudinal section of a healthy leaf displaying the epidermis (Ep), vascular bundles (Hv) and palisade parenchyma (Pe). C) Longitudinal section 24 hai with sporangia (Es) in the trichome base. D) Longitudinal section 48 hai with sporangia in the epidermis. Bar = 40 µm.

Virome of the vegetable prickly pear cactus in the central zone of Mexico. Figure 1 - Symptoms associated with virosis in vegetable prickly pear cactus cladodes. (A-D) Samples of prickly pear cactus collected in Mexico City, Milpa Alta Borough, (E) Prickly pear cactus sample collected in the State of Mexico, Otumba Municipality, (F) Healthy prickly pear cactus sample from the State of Mexico.

Figure 1 - Symptoms associated with virosis in vegetable prickly pear cactus cladodes. (A-D) Samples of prickly pear cactus collected in Mexico City, Milpa Alta Borough, (E) Prickly pear cactus sample collected in the State of Mexico, Otumba Municipality, (F) Healthy prickly pear cactus sample from the State of Mexico.

Inhibition of <em>Colletotrichum</em> spp. causing anthracnose in coffee (<em>Coffea arabica</em>) using native isolates of <em>Trichoderma</em> sp.. Figure 1 - Macroscopic characterization of 12 <em>Trichoderma</em> isolates on PDA.

Figure 1 - Macroscopic characterization of 12 Trichoderma isolates on PDA.

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Vol. 2024, 42(1)
Phytopathological Note

Etiology of rhizome rot of asparagus (Asparagus officinalis) in Atenco, Mexico State

byJuan Agustin Gonzalez Cruces*, José Sergio Sandoval Islas , Cristian Nava Díaz , Maricarmen Sandoval Sánchez

Received: 30/November/2023 – Published: 16/November/2023
DOI: https://doi.org/10.18781/R.MEX.FIT.2211-1

Abstract Background/Objective. The objective of this research was to identify the causal agent of asparagus rhizome rot, as well as evaluate different inoculation methods and the severity of the isolates.

Materials and Methods. Sampling was carried out in five producing plots Atenco, Edo. from Mexico. Five isolates of Fusarium spp. were selected. (one per plot) to perform pathogenicity tests. Three isolates were selected for their colonization characteristics for severity tests with different inoculation methods: Immersion for 12 h, immersion for 30 min and inoculation by contact with absorbent paper soaked in 1 mL of inoculum. Concentrations of 1x106 conidia mL-1 were used. 10 rhizomes were used per treatment and 10 rhizomes without inoculation. To determine the severity, photographs (in GIMP®) of the rhizome were analyzed seven days after inoculation. The isolates were molecularly identified with ITS4/ ITS5, EF688/EF1521 and TUBT1/BT2B.

Results. Fusarium prolifetatum was morphologically and molecularly identified in the three isolates. The P3DR isolate was the most severe (14.6%), followed by P5DR (13.9%) and P1SIR (11.6%).

Conclusion. The most effective inoculation method was immersion for 30 min. They were registered in the NCBI Gene Bank with accessions ON738484 (P3DR), ON973801 (P5DR) and ON738483 (P1SIR). This is the first report of F. prolifetatum in the Edo. from Mexico.

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Figure 1. Symptoms of wilting in 6-year-old asparagus crop. A) Asparagus crop with symptoms of generalized decline; B) Plant with symptoms of generalized wilting; C) Plant with symptoms of initial wilting; D) Rotting of the rhizome caused by Fusarium proliferatum; E) Basal part of the stem with symptoms of F) Basal part of the stem with vascular bundle blockage.

Figure 2. F. proliferatum isolations used for the pathogenicity tests via different inoculation methods. A) Concatenated phylogenetic tree of isolates ON738483 (PISIR), ON738484 (P3DR) and ON973801 (P5DR) with the primers ITS 4-ITS5, EF688-EF1521, TUB T1-BT2B. B) Rhizome colonization by isolate P1SIR, C) Mycelial growth of Fusarium on the front and back of PDA medium after 7 days of growth, D) Macro and microconidia at 40X. E) Rhizome colonization by isolation P3DR, F) Mycelial growth of Fusarium on the front and back of PDA medium after 7 days of growth, G) Macro and microconidia at 40X. H) Rhizome colonization by isolate P5DR, I) Mycelial growth of Fusarium on the front and back of PDA medium after 7 days of growth, J) Macro and microconidia at 40X

Figure 3. Inoculation methods with <em>Fusarium</em> isolations in asparagus rhizomes. A) Immersion of rhizome for 12 h; B) Inoculation with filter paper; C) Immersion of rhizome for 30 min, showing the rhizome suspended by disinfected mesh and moist filter paper inside a sterile square box; D) Rhizome by the immersion method for 12 h E) Rhizome by the inoculation method with filter paper F) Rhizome by the immersion method for 30 minutes

Figure 3. Inoculation methods with Fusarium isolations in asparagus rhizomes. A) Immersion of rhizome for 12 h; B) Inoculation with filter paper; C) Immersion of rhizome for 30 min, showing the rhizome suspended by disinfected mesh and moist filter paper inside a sterile square box; D) Rhizome by the immersion method for 12 h E) Rhizome by the inoculation method with filter paper F) Rhizome by the immersion method for 30 minutes

Figure 4. Percentage of severity of three F. proliferatum isolates in asparagus under three inoculation methods, and a control, not inoculated.

Table 1. Asparagus plots sampled with symptoms of decline and wilting with low technology (gravity irrigation and without mulching) inorganic agronomic management and the phenological stage of flowering-fruiting, in Atenco, State of Mexico.

Table 2. Primers used, sequence, region of the amplified gene, amplicon size, and PCR conditions for the genomic

Vol. 2023, 41(3)
Phytopathological Note

Diagrammatic scale to evaluate the severity of gray mold (Botrytis cinerea) in pomegranate

byAlberto Patricio Hernández , Yuridia Mercado Flores*, Alejandro Téllez Jurado , María del Rocío Ramírez Vargas , Andrés Quezada Salinas

Received: 30/October/2022 – Published: 15/June/2023
DOI: https://doi.org/10.18781/R.MEX.FIT.2302-9

Abstract The aim of this study was to design and validate a diagrammatic scale to estimate the severity of gray mold induced by Botrytis cinerea in pomegranate cultivation. A total of 120 healthy and diseased fruits with varying degrees of affliction were collected from orchards with active production located in the municipalities of Chilcuahutla and Taxquillo in the state of Hidalgo, Mexico (20° 18’ 11’’ N, 99° 14’ 23’’ W, 20° 32’ 01’’ N, 99° 20’ 03’’ W, respectively). From these, 60 were selected to determine the severity percentage, according to a 6-class scale (Class 0 = 0%, Class 1 = >0% - 5% - 10%, Class 2 = >10% - 25% - 50%, Class 3 = >50% - 75% - 85%, Class 4 = >85% - 90% - 95%, and Class 5 = >95% - 100%), using the 2LOG software. With the obtained data, representative images were selected to build the diagrammatic scale using Adobe Photoshop. The accuracy (r2), precision (β0), and reproducibility (β1) were verified by simple linear regression applied to the data obtained by 12 evaluators with and without experience in the observation of plant diseases. As a result, values of r2 of 0.42 and 0.85 were obtained, without and with the use of the scale, respectively, which confirmed that this tool is suitable to evaluate the severity of the disease accurately and reproducibly.

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Vol. 2023, 41(3)
Phytopathological Note

In vitro antagonism of Trichoderma against Rhizoctonia solani

byJesús Orlando Pérez González , Sergio Gavino Ramírez Rojas , Ramiro Rocha Rodríguez , Katya Ornelas Ocampo , Jorge Miguel Vázquez Alvarado , Filogonio Jesús Hernández Guzmán , Mariel Garduño Audelo*

Received: 30/October/2022 – Published: 15/June/2023
DOI: https://doi.org/10.18781/R.MEX.FIT.2304-2

Abstract Trichoderma spp., is a highly efficient antagonist of root pathogens, such as Rhizoctonia solani, which causes loss in many crops. The aim of this research was to evaluate in vitro the antagonistic capacity of T. viride, T. koningii, T. harzianum and Trichoderma spp. isolates against R. solani from a potato crop. In confrontation tests, all Trichoderma isolates were classified as antagonists class 2 according to Bell scale, where T. harzianum and T. koningii showed more than 60% inhibition of the radial growth of R. solani at 120 h. In the interaction between T. harzianum and Trichoderma spp. with R. solani, as mycoparasitism strategy, vacuolization, lysis, coiling, and penetration were demonstrated, the last two were present in all Trichoderma isolates evaluated.

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Vol. 2023, 41(3)
Review Article

Considerations about interference RNA for the control of fungal diseases in Mexican and Latin American agriculture

byOsvaldo Jhosimar Couoh Dzul , Karla Gisel Carreón Anguiano , Blondy Canto Canché*

Received: 30/October/2022 – Published: 15/June/2023
DOI: https://doi.org/10.18781/R.MEX.FIT.2210-5

Abstract The control of phytopathogens is key for food security. In the last decade, the use of interference RNA (iRNA) has been proposed as a technological tool for controlling diseases and pests in agriculture. Although different approaches have been described, such as the use of “Host-Induce Gene Silencing” (HIGS) and “Virus-Induce Gene Silencing” (VIGS), more recently a non-transgenic and environmentally friendly approach has emerged, called “Spray -Induce Gene Silencing” (SIGS), which uses double-stranded “naked” RNA (dsRNA). This review discusses recent reports on the use of dsRNA, especially SIGS, to control phytopathogenic fungi; emphasizing factors such as efficacy, safety in terms of human health and its stability in the environment. It also focuses on important phytosanitary problems in Mexico and Latin America that can be addressed with SIGS. This review concludes that SIGS technology has real potential to be used to control phytopathogenic fungi on plants in the field and on postharvest fruits. At the end, the critical tasks and the lines of research that must be carried out to promote the SIGS to make it a reality are considered.

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Vol. 2023, 41(3)
Scientific Article

Pathogenicity, virulence and in vitro sensitivity of Elsinoe perseae (= Sphaceloma perseae) isolates to different fungicides

byEdna Esquivel Miguel , José Luciano Morales García*, Martha Elena Pedraza Santos , Ana Tztzqui Chávez Bárcenas , Soledad García Morales , Samuel Pineda Guillermo

Received: 01/May/2023 – Published: 24/August/2023
DOI: https://doi.org/10.18781/R.MEX.FIT.2302-3

Abstract Elsinoe perseae (= Sphaceloma perseae) is the causal agent of the Mancha purpura or scab in avocado (Persea americana). In this study, the pathogenicity and virulence of E. perseae isolates from different agroecologicalproducing areas of Michoacán, Mexico will be reduced. For this, nursery plants with avocado fruits of Flor de María and Méndez varieties were used. On the other hand, the in vitro sensitivity of chemical fungicides (Azoxystrobin, thiabendazole, Pyraclostrobin, Cyprodinil + Fludioxonil and Azoxystrobin + Propiconazole) and authorized for use in orchards with organic management (copper sulfate, copper gluconate, copper oxychloride and the plant extract Larrea tridentata). The observed symptoms of Mancha purpura in the inoculated fruits were corroborated with those described for E. perseae in avocado. Inoculated fruits Flor de María variety shows the highest susceptibility to the pathogen. The isolates of E. perseae presented different degrees of virulence. The isolates showed different in vitro sensitivity values to the fungicides evaluated in the experiment. The pathogen showed the most sensitivity in vitro to chemical fungicides: thiabendazole and Azoxystrobin + Propiconazole (100% inhibition), and to those authorized in orchards with organic management: L. tridentata and copper oxychloride (on average 58% inhibition).

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Vol. 2023, 41(3)
Scientific Article

Effect of adjuvants, fungicides and insecticides on the growth of Trichoderma koningiopsis Th003

byYimmy Alexander Zapata Narváez*, Blanca Lucia Botina Azain

Received: 01/May/2023 – Published: 24/August/2023
DOI: https://doi.org/10.18781/R.MEX.FIT.2305-1

Abstract The effect of 44 agrochemicals (fungicides, insecticides and adjuvants) on the mycelial growth and germination conidia of Trichoderma koningiopsis Th003 was determined by seeding of 5 mm discs of fungal mycelium in Sabouraud agar supplemented with each agrochemical and seeding of conidia suspended in solutions of agrochemicals in water agar. For the adjuvants, their effect on the phyllospheric establishment of T. koningiopsis Th003 was determined by following their population in cape gooseberry leaflets inoculated with the fungus suspended in them. Eight fungicides did not inhibit the fungus mycelial growth or the conidia germination. Fenhexamid - Tebuconazole, Flutriafol and Kasugamicina inhibited it between 34 and 48% without affecting germination, Thiram - Pyrimethanil, Prochloraz, Tiabendazol, Spiroxamina and Triadimenol - Tebuconazole inhibited the growth and Thiram - Pyrimethanil and Dodine did not allow conidia germination. Insecticides and adjuvants presented an inhibition of up to 70% but did not affect the conidia germination. No negative effects of the adjuvants on the phyllosphere establishment of T. koningiopsis Th003 were observed, recovering from the treatments approximately 1x103 CFU g-1.

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Vol. 2023, 41(3)
Scientific Article

Gene silencing induced by the vector EuMY-YP∆CHS4 during the Fusarium sp. - Nicotiana benthamiana interaction

byNidia Esther Hau Yama , Marianne Soledad Luna Rivero , Blondy Beatriz Canto Canché , Yereni Minero García , Cecilia Hernández Zepeda , Diana Sánchez Rangel , Oscar Alberto Moreno Valenzuela*

Received: 10/February/2022 – Published: 31/July/2023
DOI: https://doi.org/10.18781/R.MEX.FIT.2305-4

Abstract In the literature, RNA-based interference methods have been proposed for the control of pathogenic fungi in plants, such as virus-mediated silencing (VIGS). The objective of this work was to evaluate the functionality and efficiency of the VIGS EuMV-YP∆CHS4 vector, which contains a fragment of the Fusarium sp. chitin synthase 4 (CHS4) gene sequence, and was constructed from the EuMV-YP vector:∆AV1. The VIGS vector was evaluated under two conditions in Nicotiana benthamiana plants previously inoculated with Fusarium sp. and in plants with inoculation after the inoculation of the VIGS vector. The results showed that in both treatments with the EuMV-YP∆CHS4, a delay in leaf fall during the first days of infection and a decrease in the stem damage of almost one centimeter, compared to the control infected with the fungus. In addition, a reduction of up to 30% in the relative expression of the CHS4 gene was observed. These results suggest that the application of VIGS vectors may be efficient as a strategy to protect plants against Fusarium sp.

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Vol. 2023, 41(3)
Scientific Article

Identification of species and physiological races of Xanthomonas isolated from tomato (Solanum lycopersicum) and chili pepper (Capsicum annuum) in Sinaloa, Mexico

byLaura Belén Tapia de la Barrera , Manuel Alonzo Báez Sañudo , Raymundo Saúl García Estrada , Juan Manuel Tovar Pedraza , José Armando Carrillo Fasio

Received: 10/February/2022 – Published: 31/July/2023
DOI: https://doi.org/10.18781/R.MEX.FIT.2210-2

Abstract Bacterial spot of tomato and chili pepper, caused by four species of Xanthomonas and various races, is one of the diseases with the greatest impact on horticulture worldwide. The aim of this study was to identify the species and physiological races of Xanthomonas present in tomato (Solanum lycopersicum) and chili pepper (Capsicum annuum) crops in Sinaloa, Mexico. For this purpose, samples with typical symptoms of bacterial spot were collected in commercial fields of the different municipalities in the state of Sinaloa. Ninety-three bacteria were isolated on semi-selective medium as nutrient agar and yeast extract-dextrose. A total of 47 bacteria were identified as Xanthomonas by a combination of morphological, pathogenic, biochemical, physiological, and molecular tests. In addition, to characterize the morphological race of each strain, differential tomato (four) and chili pepper (six) lines were used. Molecular diagnostic using specific primers indicated that 83% corresponded to X. euvesicatoria, 10.6% to X. perforans, and 6.4% to X. vesicatoria. At the physiological race level, races T1, T2, T3, and T5 were detected in tomato; while the Xanthomonas races detected in chili pepper were P0, P3, P6, P8 and P10. This information updates previous data on the distribution of races of Xanthomonas that infect chili peppers in Sinaloa, since races P6 and P10 are reported for the first time.

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Figure 1. Agarose gel to detect <em>Xanthomonas</em> spp. species. In the first lane (M) 100 pb marker; in the second lane, primers for X. euvesicatoria (E); third, X. vesicatoria (V), fourth lane with a specific stripe for X. perforans (P); fifth, X. gardneri (G) and sixth lane with universal primers for <em>Xanthomonas</em> spp. (X)

Figure 1. Agarose gel to detect Xanthomonas spp. species. In the first lane (M) 100 pb marker; in the second lane, primers for X. euvesicatoria (E); third, X. vesicatoria (V), fourth lane with a specific stripe for X. perforans (P); fifth, X. gardneri (G) and sixth lane with universal primers for Xanthomonas spp. (X)

Table 1. Specific primers for the identification of <em>Xanthomonas</em> species in strains obtained from tomato and chili pepper in Sinaloa, Mexico

Table 1. Specific primers for the identification of Xanthomonas species in strains obtained from tomato and chili pepper in Sinaloa, Mexico

Table 2. Reaction mixture for PCR for the identification of <em>Xanthomonas</em> species

Table 2. Reaction mixture for PCR for the identification of Xanthomonas species

Table 3. Classification of <em>Xanthomonas</em> spp. races in chili pepper (<em>Capsicum annuum</em>) by susceptibility and hypersensitivity reactions.

Table 3. Classification of Xanthomonas spp. races in chili pepper (Capsicum annuum) by susceptibility and hypersensitivity reactions.

Table 4. Classification of physiological <em>Xanthomonas</em> races in tomato by susceptibility and hypersensitivity reactions.

Table 4. Classification of physiological Xanthomonas races in tomato by susceptibility and hypersensitivity reactions.

Table 5. Species and races of <em>Xanthomonas</em> isolated from chili pepper and tomato from Sinaloa from November, 2016 to March, 2017.

Table 5. Species and races of Xanthomonas isolated from chili pepper and tomato from Sinaloa from November, 2016 to March, 2017.

Vol. 2023, 41(3)
Scientific Article

Alternatives for the gray mold (Botrytis cinerea) control in cape gooseberry (Physalis peruviana) crop

byYimmy Alexander Zapata Narváez*, Andrés Díaz Garcia , Camilo Rubén Beltrán Acosta

Received: 10/February/2022 – Published: 31/July/2023
DOI: https://doi.org/10.18781/R.MEX.FIT.2302-5

Abstract The effect of the field applications of three bioproducts (based on Trichoderma koningiopsis, Rhodotorula mucilaginosa, and Bacillus amyloliquefaciens), the alternation of the biostimulant Kendal® and the Swinglea glutinosa extract and the rotations of two fungicides (based on Azoxystrobin-Difenoconazole and Thiram- Pyrimethanol), on the incidence of gray mold in cape gooseberry postharvest was evaluated. For this purpose, the fruit was harvested weekly in the field, arranged in wet chambers with fruits with and without calyx, and incubated for seven days at 20 °C in laboratory conditions to promote the development of B. cinerea and determine the efficacy in its control. In addition, the populations of the microbial antagonists were monitored between applications by collecting the leaflets and washing them in 0.1% Tween 80 and sowing aliquots in specific culture media. In fruits with calyx, the lowest incidence of the gray mold, with averages of 48 and 51%, occurred with the applications of the bioproducts based on T. koningiopsis and R. mucilaginosa, respectively. In contrast, the incidence did not exceed 1.4% in fruits without calyx in all treatments. Furthermore, the population of microbial antagonists in the phyllosphere remained constant between applications, with counts of 1x103 CFU g-1 for T. koningiopsis and 1x105 CFU g-1 for R. mucilaginosa and B. amyloliquefaciens.

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Figure 1. Top, healthy cape gooseberry (<em>Physalis peruviana</em>) fruits. Below, fruits with signs of gray mold (<em>Botrytis cinerea</em>) in the field, showing the presence of the mycelia and conidia of <em>Botrytis cinerea</em> with a grayish tome on the calyx and the berry inside.

Figure 1. Top, healthy cape gooseberry (Physalis peruviana) fruits. Below, fruits with signs of gray mold (Botrytis cinerea) in the field, showing the presence of the mycelia and conidia of Botrytis cinerea with a grayish tome on the calyx and the berry inside.

Table 1. Treatments evaluated for the control of gray mold (<em>Botrytis cinerea</em>) in the cape gooseberry crop.

Table 1. Treatments evaluated for the control of gray mold (Botrytis cinerea) in the cape gooseberry crop.

Vol. 2023, 41(3)
Scientific Article

Biodegradables films with fungistatic activity for the postharvest control of Fusarium solani in Hass avocado fruit

byWendy Abril Flores Pérez , Nemesio Villa Ruano , Efraín Rubio Rosas*

Received: 31/January/2022 – Published: 23/August/2023
DOI: https://doi.org/10.18781/R.MEX.FIT.2303-4

Abstract Little is known on the use of biodegradable films for the control of diseases in crops of agricultural importance. Consequently, the objective of this work was to determine the efficacy of chitosan hybrid films impregnated with thyme essential oil on Hass avocado fruits previously infected with Fusarium solani isolated from the northeastern highlands of Puebla, Mexico. The native strain of F. solani was morphologically and molecularly identified and the fungistatic activity of three chitosan films supplemented with 0.7% (FT1), 1.0% (FT2) and 1.3% (FT3) of thyme essential oil was evaluated in situ on the growth of F. solani in avocado fruits. Texture, transmittance and opacity of the films were obtained by scanning electron microscopy and UV-Vis spectrometry, respectively. Overall, it was recorded that films with a higher concentration of thyme essential oil (1-1.3% w/v) presented lower transmittance in the UV light range and higher opacity. Avocado fruits infected with F. solani simultaneously treated with FT2 and FT3 reduced the appearance of symptoms while preserved firmness, as well as fiber, fat, reducing sugars, and protein content (p < 0.01). In the same context, these materials promoted the conservation of the content of the nutraceuticals linoleic acid, oleic acid, palmitic acid and palmitoleic acid for 21 days. These results suggest that the hybrid films generated in the present study have the ability to control fusariosis caused by this fungus, prolonging the shelf life of Hass avocado fruit.

Show Figures and/or Tables

Figure 1. Morphology of Fusariun solani. A) Radial growth observed from the obverse of Petri dish in PDA after 7 days. B) Radial growth observed from the reverse of Petri dish. C) Macroconida observed at 50X without staining. D) Microconidia observed at 50X stained with cotton blue.

Figure 2. Phylogenetic tree of <em>Fusarium solani</em> with concatenated sequences of ITS and EF-1α. The strain <em>F. solani</em> isolated from the northeast highland of Puebla is remarked in bold font.

Figure 2. Phylogenetic tree of Fusarium solani with concatenated sequences of ITS and EF-1α. The strain F. solani isolated from the northeast highland of Puebla is remarked in bold font.

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